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macrophage colony  (MedChemExpress)


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    Structured Review

    MedChemExpress macrophage colony
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Macrophage Colony, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m+csf/M-CSF%2C+Mouse/pmc13471083-120-17-23
    Average 98 stars, based on 35 article reviews
    macrophage colony - by Bioz Stars, 2026-09
    98/100 stars

    Images

    1) Product Images from "APOE -associated lipid-handling macrophages in hepatocellular carcinoma: ligand–receptor communication and host Apoe -linked myeloid remodeling"

    Article Title: APOE -associated lipid-handling macrophages in hepatocellular carcinoma: ligand–receptor communication and host Apoe -linked myeloid remodeling

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2026.1902143

    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, macrophage colony-stimulating factor; BMDMs, bone marrow-derived macrophages.
    Figure Legend Snippet: Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, macrophage colony-stimulating factor; BMDMs, bone marrow-derived macrophages.

    Techniques Used: Isolation, Staining, Flow Cytometry, Fluorescence, Two Tailed Test, Derivative Assay

    Related Articles

    Clinical Proteomics:

    Article Title: m6A‐Modified KLF11 Inhibits Macrophage Foam Cell Formation by Transcriptionally Suppressing ATP5B Expression in Atherosclerosis
    Article Snippet: Macrophage foam cell formation is the mark of atherosclerosis (AS).. Krüppellike factor (KLF)11 prevented the development of AS in diabetic conditions.. However, it is not clear whether KLF11 inhibits macrophage foam cell formation.

    Cell Culture:

    Article Title: Inhalable extracellular vesicle delivered IL-10 mRNA attenuates pulmonary hypertension in rats.
    Article Snippet: .. The BMMs were cultured in DMEM supplemented with 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin, along with 20 ng/mL of M-CSF (MedChemExpress), for 7 days before further treatment. ..

    Article Title: A glycopeptide hydrogel confers protection and treatment in sepsis via recruitment and training of macrophages
    Article Snippet: Mouse macrophages RAW264.7 were purchased from ATCC (USA) and cultured in RPMI-1640 (Gibco, Grand Island, NY) medium containing 10% fetal bovine serum (FBS, Gibco) and 1% Penicillin-Streptomycin (Gibco) at 37 °C in 5% CO2. .. Bone marrow-derived macrophages (BMDMs) were extracted from the tibia of C57BL/6 mice aged 6-8 weeks and cultured 5 days in RPMI-1640 medium containing 20 ng/mL M-CSF (MCE, New Jersey, USA) by adaptation of the previous procedures. .. Escherichia coli ( E. coli ) was donated by Nankai University, and E. coli fluorescent strain (ATCC 25922) was purchased from Baosai Biotechnology Co., Ltd. (Hangzhou, China), and was cultured in soybean agar containing tryptone at 37 °C.

    other:

    Article Title: VG161 oncolytic virus remodels the tumor microenvironment by expanding CD3 + macrophages and enhancing antitumor immunity.
    Article Snippet: Oncolytic virotherapy has emerged as a promising strategy for cancer immunotherapy; however, its immunomodulatory mechanisms in colorectal cancer remain incompletely understood.. Here, we evaluated the therapeutic effects of the oncolytic virus VG161 in murine colorectal cancer models (CT26.WT and MC38) and systematically characterized the associated tumor microenvironment (TME) remodeling.. Using single-cell RNA sequencing, flow cytometry, and multiplex immunohistochemistry, we identified a distinct population of CD3 macrophages that was markedly expanded following VG161 treatment.

    Recombinant:

    Article Title: Monocyte-derived LGMN⁺ macrophages divert lung injury outcomes toward fibrosis through matrix remodeling
    Article Snippet: Cultures were maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), 10 mM HEPES, and 2 mM GlutaMAX (all Gibco). .. To induce differentiation into macrophages, recombinant human GM-CSF or M-CSF (MedChemExpress; 10 ng/mL) was added immediately after plating. ..

    Isolation:

    Article Title: Targeting the tumor microenvironment: reprogramming macrophages as a novel therapeutic strategy in FUOM-deficient glioblastoma
    Article Snippet: Subsequently, the derived M0 macrophages were further activated by LPS (100 ng/mL, L2630, Sigma, DE) + INF-γ (20 ng/mL, AF-300-02, Peprotech, USA) or IL-4 (30 ng/mL, 200-04-5ug, Peprotech, USA) + IL-13 (30 ng/mL, 200-13-2ug, Peprotech, USA) for 48 h, polarized into M1- or M2-like macrophages respectively. .. Bone marrow cells were isolated from the femurs and tibias of mice and differentiated into bone marrow-derived macrophages (BMDMs) using M-CSF(HY-P7085, MCE, SH, China). ..



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    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
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    Image Search Results


    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, macrophage colony-stimulating factor; BMDMs, bone marrow-derived macrophages.

    Journal: Frontiers in Immunology

    Article Title: APOE -associated lipid-handling macrophages in hepatocellular carcinoma: ligand–receptor communication and host Apoe -linked myeloid remodeling

    doi: 10.3389/fimmu.2026.1902143

    Figure Lengend Snippet: Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, macrophage colony-stimulating factor; BMDMs, bone marrow-derived macrophages.

    Article Snippet: Bone marrow cells from WT and Apoe −/− mice were differentiated into bone marrow-derived macrophages (BMDMs) with macrophage colony-stimulating factor (M-CSF; 25 ng/mL; MedChemExpress, HY-P7085) for 7 days.

    Techniques: Isolation, Staining, Flow Cytometry, Fluorescence, Two Tailed Test, Derivative Assay